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Antibiotics will not affect a pregnancy test...I was on antibiotics for a UTI and did some research to see if it would cause inaccurate results for HPT's and they don't...my doc even had me do a urine test when I went in for my yearly exam a few days ago and she knew I was on antibiotics...the only medications that do affect HPT's are the ones with the HCG hormone in them. Did your doc or someone tell you they would affect them?